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PsychedeliaDokholyan Laboratory
TargetEnzymes

N-acylethanolamine-hydrolyzing acid amidase

Named enzymes, including the metabolising cytochromes and the monoamine oxidases.

Measurements
16
Compounds
8
Binding constants
0
Median pX
—

I · Identity

What this target is

Its function as UniProt states it, where in a cell the protein is found, and which cell types and tissues transcribe its gene.

Protein

NAAA_HUMANNAAAHomo sapiens

N-acylethanolamine-hydrolyzing acid amidase

Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N-palmitoylethanolamine > N-myristoylethanolamine > N-lauroylethanolamine = N-stearoylethanolamine > N-arachidonoylethanolamine > N-oleoylethanolamine15655246,17980170,18793752,22825852,30301806. Also exhibits weak hydrolytic activity against the ceramides N-lauroylsphingosine and N-palmitoylsphingosine15655246

Quoted from UniProt’s FUNCTION annotation for Q02083 (reviewed). The superscript numbers are UniProt’s own PubMed citations, kept in the sentence and in order, each linking to the paper it names.

What it acts with

STRING’s highest-confidence human functional partners. The combined score mixes several kinds of evidence, so each channel is shown separately — a partner supported by experiment is a different claim from one supported only by text mining, and for a receptor this is where its coupling appears as evidence rather than as assertion.

PartnerCombinedExperimentDatabaseText mining
FAAH0.8500.0490.0000.847
FIG40.8400.0000.0000.839
NAPEPLD0.8160.0000.0000.816
MGLL0.7800.2720.0000.710
LAMP20.7780.0000.0000.772
VAC140.7600.0000.0000.760
FAAH20.7510.0490.0000.749
PPARA0.7020.0000.0000.702
ABHD40.6610.0000.0000.657
GPR550.6600.0000.0000.660

Where it sits in the cell

UniProt, curated Lysosome · Membrane

Expression by cell type

Single cell type RNA, the 6 highest of those the Human Protein Atlas reports for this gene. Transcript, not protein, and a cell type rather than a tissue.

  • Breast lactating cells322.2 nCPM
  • Prostatic glandular cells265.8 nCPM
  • Enterocytes233.2 nCPM
  • Kupffer cells170.1 nCPM
  • Colonocytes161.9 nCPM
  • monocytes157.1 nCPM

II · Anatomy

Where the gene is transcribed

The same two atlases the compound plates use, asked about this gene alone. Brain on the left, peripheral and autonomic tissues on the right.

Brain

cerebral cortex — 7.9 nTPM, from NAAAcerebellum — 10.9 nTPM, from NAAAhippocampal formation — 7.7 nTPM, from NAAAamygdala — 6.4 nTPM, from NAAAhypothalamus — 9.9 nTPM, from NAAAthalamus — no resolved target expressed herebasal ganglia — 10.2 nTPM, from NAAAmedulla oblongata — no resolved target expressed here

basal ganglia: drawn as the caudate nucleus, putamen and globus pallidus.

Measured but not drawn — this anatomogram has no outline for them: retina 30.3, spinal cord 11.3, midbrain 8.3, pituitary gland 7.4.

Body

heart — 18.6 nTPM, from NAAAsmooth muscle — 14.3 nTPM, from NAAAlung — 21.7 nTPM, from NAAAliver — 63.9 nTPM, from NAAAkidney — 37.9 nTPM, from NAAAstomach — 17.4 nTPM, from NAAAduodenum — 28.9 nTPM, from NAAAsmall intestine — 57.1 nTPM, from NAAAcolon — 57.8 nTPM, from NAAAadrenal gland — 13.7 nTPM, from NAAAthyroid gland — 14.9 nTPM, from NAAApancreas — 7.1 nTPM, from NAAAurinary bladder — 18.7 nTPM, from NAAAskeletal muscle — 8.1 nTPM, from NAAAspleen — 59.9 nTPM, from NAAAsalivary gland — 30.4 nTPM, from NAAA
064 nTPM

Square-root ramp · shared scale across both plates

Shading is transcript level — not affinity, and not affinity combined with expression. Outlined but unshaded organs hold none of NAAA transcript above the atlas’s detection threshold. Anatomy from EMBL-EBI Anatomogram (Apache License 2.0); regions identified by Uberon (uberon.org), CC BY 3.0.

Brain · 10

  • retina30.3 nTPM
  • spinal cord11.3 nTPM
  • cerebellum10.9 nTPM
  • basal ganglia10.2 nTPM
  • hypothalamus9.9 nTPM
  • midbrain8.3 nTPM
  • cerebral cortex7.9 nTPM
  • hippocampal formation7.7 nTPM
  • pituitary gland7.4 nTPM
  • amygdala6.4 nTPM

Peripheral and autonomic · 16

  • liver63.9 nTPM
  • spleen59.9 nTPM
  • colon57.8 nTPM
  • small intestine57.1 nTPM
  • kidney37.9 nTPM
  • salivary gland30.4 nTPM
  • duodenum28.9 nTPM
  • lung21.7 nTPM
  • urinary bladder18.7 nTPM
  • heart muscle18.6 nTPM
  • stomach 117.4 nTPM
  • thyroid gland14.9 nTPM
  • smooth muscle14.3 nTPM
  • adrenal gland13.7 nTPM
  • skeletal muscle8.1 nTPM
  • pancreas7.1 nTPM

Transcript is not protein. These are RNA levels for NAAA, shaded on √nTPM so that a three-order range stays legible — on a linear ramp every region but the strongest would read as blank, which looks like absence rather than less. A region carrying no shading is one the atlas reports no consensus value for, not one where the gene is silent. And a gene being transcribed somewhere is not evidence that any compound reaches it. Human Protein Atlas, RNA expression, nTPM (normalized transcripts per million), consensus tissue dataset.

IV · Evidence

Every measurement at this target

Each row links to the ChEMBL assay it came from.

Functional readouts 8

IC₅₀, EC₅₀, AC₅₀ and potency. Assay-dependent, and not interchangeable with a binding constant.

TargetClassTypeValue (nM)pXOrganismAssay
N-acylethanolamine-hydrolyzing acid amidaseEnzymesIC50— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424982
N-acylethanolamine-hydrolyzing acid amidaseEnzymesIC50— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424982
N-acylethanolamine-hydrolyzing acid amidaseEnzymesIC50— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424982
N-acylethanolamine-hydrolyzing acid amidaseEnzymesIC50— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424982
N-acylethanolamine-hydrolyzing acid amidaseEnzymesIC50— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424982
N-acylethanolamine-hydrolyzing acid amidaseEnzymesIC50— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424982
N-acylethanolamine-hydrolyzing acid amidaseEnzymesIC50— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424982
N-acylethanolamine-hydrolyzing acid amidaseEnzymesIC50— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424982

Other measurements 8

Everything else ChEMBL recorded for this compound — percentage inhibition, efficacy, half-life, cytotoxicity. Reported in the assay's own units, which is why no value is converted here.

TargetClassTypeValue (nM)pXOrganismAssay
N-acylethanolamine-hydrolyzing acid amidaseEnzymesEmax— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424984
N-acylethanolamine-hydrolyzing acid amidaseEnzymesEmax— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424984
N-acylethanolamine-hydrolyzing acid amidaseEnzymesEmax— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424984
N-acylethanolamine-hydrolyzing acid amidaseEnzymesEmax— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424984
N-acylethanolamine-hydrolyzing acid amidaseEnzymesEmax— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424984
N-acylethanolamine-hydrolyzing acid amidaseEnzymesEmax— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424983
N-acylethanolamine-hydrolyzing acid amidaseEnzymesEmax— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424983
N-acylethanolamine-hydrolyzing acid amidaseEnzymesEmax— no normalized value— value does not convert to pXHomo sapiensCHEMBL4424983
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