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PsychedeliaDokholyan Laboratory
TargetEnzymes

Dual specificity protein kinase CLK4

Named enzymes, including the metabolising cytochromes and the monoamine oxidases.

Measurements
7
Compounds
1
Binding constants
0
Median pX
—

I · Identity

What this target is

Its function as UniProt states it, where in a cell the protein is found, and which cell types and tissues transcribe its gene.

Protein

CLK4_HUMANCLK4Homo sapiens

Dual specificity protein kinase CLK4

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells

Quoted from UniProt’s FUNCTION annotation for Q9HAZ1 (reviewed).

What it acts with

STRING’s highest-confidence human functional partners. The combined score mixes several kinds of evidence, so each channel is shown separately — a partner supported by experiment is a different claim from one supported only by text mining, and for a receptor this is where its coupling appears as evidence rather than as assertion.

PartnerCombinedExperimentDatabaseText mining
CLK10.9590.0000.9000.056
MBP0.6580.0000.0000.658
SRPK10.5880.4310.0000.305
SRSF60.5830.1730.0000.494
SRSF50.5580.1740.0000.434
SRSF40.5580.1990.0000.452
H2BC210.5570.0000.0000.549
UBL50.5510.4740.0000.181
PNISR0.5400.0700.0000.166
JMJD60.5240.5240.0000.000

Where it sits in the cell

  • Actin filaments

Human Protein Atlas, by antibody immunofluorescence in cell lines — a different assay from the expression figures below, which are RNA.

UniProt, curated Nucleus

Expression by cell type

Single cell type RNA, the 2 highest of those the Human Protein Atlas reports for this gene. Transcript, not protein, and a cell type rather than a tissue.

  • Late spermatids773.4 nCPM
  • Early spermatids355.3 nCPM

II · Anatomy

Where the gene is transcribed

The same two atlases the compound plates use, asked about this gene alone. Brain on the left, peripheral and autonomic tissues on the right.

Brain

cerebral cortex — 11.3 nTPM, from CLK4cerebellum — 53.5 nTPM, from CLK4hippocampal formation — 13.1 nTPM, from CLK4amygdala — 10.4 nTPM, from CLK4hypothalamus — 9.6 nTPM, from CLK4thalamus — no resolved target expressed herebasal ganglia — 12.0 nTPM, from CLK4medulla oblongata — no resolved target expressed here

basal ganglia: drawn as the caudate nucleus, putamen and globus pallidus.

Measured but not drawn — this anatomogram has no outline for them: retina 30.8, spinal cord 14.6, midbrain 11.8, pituitary gland 10.4.

Body

heart — 12.7 nTPM, from CLK4smooth muscle — 5.1 nTPM, from CLK4lung — 10.5 nTPM, from CLK4liver — 13.7 nTPM, from CLK4kidney — 13.6 nTPM, from CLK4stomach — 12.1 nTPM, from CLK4duodenum — 8.7 nTPM, from CLK4small intestine — 12.4 nTPM, from CLK4colon — 14.3 nTPM, from CLK4adrenal gland — 11.0 nTPM, from CLK4thyroid gland — 16.5 nTPM, from CLK4pancreas — 17.4 nTPM, from CLK4urinary bladder — 12.7 nTPM, from CLK4skeletal muscle — 16.4 nTPM, from CLK4spleen — 18.4 nTPM, from CLK4salivary gland — 12.7 nTPM, from CLK4
054 nTPM

Square-root ramp · shared scale across both plates

Shading is transcript level — not affinity, and not affinity combined with expression. Outlined but unshaded organs hold none of CLK4 transcript above the atlas’s detection threshold. Anatomy from EMBL-EBI Anatomogram (Apache License 2.0); regions identified by Uberon (uberon.org), CC BY 3.0.

Brain · 10

  • cerebellum53.5 nTPM
  • retina30.8 nTPM
  • spinal cord14.6 nTPM
  • hippocampal formation13.1 nTPM
  • basal ganglia12.0 nTPM
  • midbrain11.8 nTPM
  • cerebral cortex11.3 nTPM
  • amygdala10.4 nTPM
  • pituitary gland10.4 nTPM
  • hypothalamus9.6 nTPM

Peripheral and autonomic · 16

  • spleen18.4 nTPM
  • pancreas17.4 nTPM
  • thyroid gland16.5 nTPM
  • skeletal muscle16.4 nTPM
  • colon14.3 nTPM
  • liver13.7 nTPM
  • kidney13.6 nTPM
  • heart muscle12.7 nTPM
  • urinary bladder12.7 nTPM
  • salivary gland12.7 nTPM
  • small intestine12.4 nTPM
  • stomach 112.1 nTPM
  • adrenal gland11.0 nTPM
  • lung10.5 nTPM
  • duodenum8.7 nTPM
  • smooth muscle5.1 nTPM

Transcript is not protein. These are RNA levels for CLK4, shaded on √nTPM so that a three-order range stays legible — on a linear ramp every region but the strongest would read as blank, which looks like absence rather than less. A region carrying no shading is one the atlas reports no consensus value for, not one where the gene is silent. And a gene being transcribed somewhere is not evidence that any compound reaches it. Human Protein Atlas, RNA expression, nTPM (normalized transcripts per million), consensus tissue dataset.

IV · Evidence

Every measurement at this target

Each row links to the ChEMBL assay it came from.

Functional readouts 4

IC₅₀, EC₅₀, AC₅₀ and potency. Assay-dependent, and not interchangeable with a binding constant.

TargetClassTypeValue (nM)pXOrganismAssay
Dual specificity protein kinase CLK4EnzymesIC5024.007.62Homo sapiensCHEMBL5235543
Dual specificity protein kinase CLK4EnzymesIC5050.007.30Mus musculusCHEMBL2182296
Dual specificity protein kinase CLK4EnzymesPotency— no normalized value— value does not convert to pXHomo sapiensCHEMBL1614024
Dual specificity protein kinase CLK4EnzymesIC50— no normalized value— value does not convert to pXHomo sapiensCHEMBL5251368

Other measurements 3

Everything else ChEMBL recorded for this compound — percentage inhibition, efficacy, half-life, cytotoxicity. Reported in the assay's own units, which is why no value is converted here.

TargetClassTypeValue (nM)pXOrganismAssay
Dual specificity protein kinase CLK4EnzymesActivity— no normalized value— value does not convert to pXHomo sapiensCHEMBL2186711
Dual specificity protein kinase CLK4EnzymesActivity— no normalized value— value does not convert to pXHomo sapiensCHEMBL4344394
Dual specificity protein kinase CLK4EnzymesActivity— no normalized value— value does not convert to pXHomo sapiensCHEMBL4151450
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