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PsychedeliaDokholyan Laboratory
TargetEnzymes

DNA polymerase kappa

Named enzymes, including the metabolising cytochromes and the monoamine oxidases.

Measurements
4
Compounds
4
Binding constants
0
Median pX
—

I · Identity

What this target is

Its function as UniProt states it, where in a cell the protein is found, and which cell types and tissues transcribe its gene.

Protein

POLK_HUMANPOLKHomo sapiens

DNA polymerase kappa

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity

Quoted from UniProt’s FUNCTION annotation for Q9UBT6 (reviewed).

What it acts with

STRING’s highest-confidence human functional partners. The combined score mixes several kinds of evidence, so each channel is shown separately — a partner supported by experiment is a different claim from one supported only by text mining, and for a receptor this is where its coupling appears as evidence rather than as assertion.

PartnerCombinedExperimentDatabaseText mining
PCNA0.9980.9940.5000.281
REV10.9840.9330.5000.462
TP530.9180.1680.9000.097
REV3L0.9100.3230.5000.716
MAD2L20.8710.5000.5000.513
RAD510.8400.2950.4000.604
POLD10.8230.5000.4000.432
POLH0.7980.0790.4000.577
POLI0.7690.0790.5000.419
POLD20.7540.0910.4000.555

Where it sits in the cell

  • Nucleoplasm

Human Protein Atlas, by antibody immunofluorescence in cell lines — a different assay from the expression figures below, which are RNA.

UniProt, curated Nucleus

II · Anatomy

Where the gene is transcribed

The same two atlases the compound plates use, asked about this gene alone. Brain on the left, peripheral and autonomic tissues on the right.

Brain

cerebral cortex — 2.2 nTPM, from POLKcerebellum — 3.7 nTPM, from POLKhippocampal formation — 2.9 nTPM, from POLKamygdala — 2.5 nTPM, from POLKhypothalamus — 2.5 nTPM, from POLKthalamus — no resolved target expressed herebasal ganglia — 3.0 nTPM, from POLKmedulla oblongata — no resolved target expressed here

basal ganglia: drawn as the caudate nucleus, putamen and globus pallidus.

Measured but not drawn — this anatomogram has no outline for them: retina 4.9, spinal cord 4.1, midbrain 3.3, pituitary gland 3.2.

Body

heart — 3.8 nTPM, from POLKsmooth muscle — 2.5 nTPM, from POLKlung — 3.9 nTPM, from POLKliver — 3.3 nTPM, from POLKkidney — 3.3 nTPM, from POLKstomach — 3.3 nTPM, from POLKduodenum — 2.2 nTPM, from POLKsmall intestine — 3.0 nTPM, from POLKcolon — 3.0 nTPM, from POLKadrenal gland — 4.1 nTPM, from POLKthyroid gland — 4.4 nTPM, from POLKpancreas — 4.1 nTPM, from POLKurinary bladder — 2.9 nTPM, from POLKskeletal muscle — 4.2 nTPM, from POLKspleen — 3.8 nTPM, from POLKsalivary gland — 3.1 nTPM, from POLK
05 nTPM

Square-root ramp · shared scale across both plates

Shading is transcript level — not affinity, and not affinity combined with expression. Outlined but unshaded organs hold none of POLK transcript above the atlas’s detection threshold. Anatomy from EMBL-EBI Anatomogram (Apache License 2.0); regions identified by Uberon (uberon.org), CC BY 3.0.

Brain · 10

  • retina4.9 nTPM
  • spinal cord4.1 nTPM
  • cerebellum3.7 nTPM
  • midbrain3.3 nTPM
  • pituitary gland3.2 nTPM
  • basal ganglia3.0 nTPM
  • hippocampal formation2.9 nTPM
  • amygdala2.5 nTPM
  • hypothalamus2.5 nTPM
  • cerebral cortex2.2 nTPM

Peripheral and autonomic · 16

  • thyroid gland4.4 nTPM
  • skeletal muscle4.2 nTPM
  • adrenal gland4.1 nTPM
  • pancreas4.1 nTPM
  • lung3.9 nTPM
  • heart muscle3.8 nTPM
  • spleen3.8 nTPM
  • liver3.3 nTPM
  • kidney3.3 nTPM
  • stomach 13.3 nTPM
  • salivary gland3.1 nTPM
  • small intestine3.0 nTPM
  • colon3.0 nTPM
  • urinary bladder2.9 nTPM
  • smooth muscle2.5 nTPM
  • duodenum2.2 nTPM

Transcript is not protein. These are RNA levels for POLK, shaded on √nTPM so that a three-order range stays legible — on a linear ramp every region but the strongest would read as blank, which looks like absence rather than less. A region carrying no shading is one the atlas reports no consensus value for, not one where the gene is silent. And a gene being transcribed somewhere is not evidence that any compound reaches it. Human Protein Atlas, RNA expression, nTPM (normalized transcripts per million), consensus tissue dataset.

IV · Evidence

Every measurement at this target

Each row links to the ChEMBL assay it came from.

Functional readouts 4

IC₅₀, EC₅₀, AC₅₀ and potency. Assay-dependent, and not interchangeable with a binding constant.

TargetClassTypeValue (nM)pXOrganismAssay
DNA polymerase kappaEnzymesPotency— no normalized value— value does not convert to pXHomo sapiensCHEMBL1794536
DNA polymerase kappaEnzymesPotency— no normalized value— value does not convert to pXHomo sapiensCHEMBL1794536
DNA polymerase kappaEnzymesPotency— no normalized value— value does not convert to pXHomo sapiensCHEMBL1794536
DNA polymerase kappaEnzymesPotency— no normalized value— value does not convert to pXHomo sapiensCHEMBL1794536
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