Skip to content
PsychedeliaDokholyan Laboratory
TargetEnzymes

DNA polymerase iota

Named enzymes, including the metabolising cytochromes and the monoamine oxidases.

Measurements
7
Compounds
7
Binding constants
0
Median pX
—

I · Identity

What this target is

Its function as UniProt states it, where in a cell the protein is found, and which cell types and tissues transcribe its gene.

Protein

POLI_HUMANPOLIHomo sapiens

DNA polymerase iota

Error-prone DNA polymerase specifically involved in DNA repair11013228,11387224. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls11013228,11387224,14630940,15199127,20388628. Favors Hoogsteen base-pairing in the active site15254543. Inserts the correct base with high-fidelity opposite an adenosine template15254543. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine11013228. May play a role in hypermutation of immunoglobulin genes12410315. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity11251121,14630940

Quoted from UniProt’s FUNCTION annotation for Q9UNA4 (reviewed). The superscript numbers are UniProt’s own PubMed citations, kept in the sentence and in order, each linking to the paper it names.

What it acts with

STRING’s highest-confidence human functional partners. The combined score mixes several kinds of evidence, so each channel is shown separately — a partner supported by experiment is a different claim from one supported only by text mining, and for a receptor this is where its coupling appears as evidence rather than as assertion.

PartnerCombinedExperimentDatabaseText mining
PCNA0.9980.9950.5000.373
RRN30.9970.0000.0000.997
UBC0.9900.9730.5000.339
REV10.9870.9050.5000.721
RPS27A0.9690.9320.5000.154
UBB0.9620.9260.5000.048
UBTF0.9620.0000.0000.962
TP530.9600.7360.0000.854
SIRT70.9550.0000.0000.955
REV3L0.9540.7570.5000.624

Where it sits in the cell

  • Nuclear speckles
  • Cytoplasmic bodiesadditional

Human Protein Atlas, by antibody immunofluorescence in cell lines — a different assay from the expression figures below, which are RNA.

UniProt, curated Nucleus

Expression by cell type

Single cell type RNA, the 3 highest of those the Human Protein Atlas reports for this gene. Transcript, not protein, and a cell type rather than a tissue.

  • Late primary spermatocytes148.5 nCPM
  • Early spermatids93.3 nCPM
  • Sertoli cells92.7 nCPM

II · Anatomy

Where the gene is transcribed

The same two atlases the compound plates use, asked about this gene alone. Brain on the left, peripheral and autonomic tissues on the right.

Brain

cerebral cortex — 8.7 nTPM, from POLIcerebellum — 4.8 nTPM, from POLIhippocampal formation — 4.9 nTPM, from POLIamygdala — 4.8 nTPM, from POLIhypothalamus — 5.3 nTPM, from POLIthalamus — no resolved target expressed herebasal ganglia — 4.7 nTPM, from POLImedulla oblongata — no resolved target expressed here

basal ganglia: drawn as the caudate nucleus, putamen and globus pallidus.

Measured but not drawn — this anatomogram has no outline for them: retina 23.5, pituitary gland 10.5, spinal cord 7.6, midbrain 5.7.

Body

heart — 10.0 nTPM, from POLIsmooth muscle — 10.7 nTPM, from POLIlung — 9.4 nTPM, from POLIliver — 7.0 nTPM, from POLIkidney — 13.4 nTPM, from POLIstomach — 9.1 nTPM, from POLIduodenum — 7.4 nTPM, from POLIsmall intestine — 6.2 nTPM, from POLIcolon — 8.8 nTPM, from POLIadrenal gland — 8.6 nTPM, from POLIthyroid gland — 29.3 nTPM, from POLIpancreas — 12.7 nTPM, from POLIurinary bladder — 9.7 nTPM, from POLIskeletal muscle — 5.8 nTPM, from POLIspleen — 8.9 nTPM, from POLIsalivary gland — 7.7 nTPM, from POLI
029 nTPM

Square-root ramp · shared scale across both plates

Shading is transcript level — not affinity, and not affinity combined with expression. Outlined but unshaded organs hold none of POLI transcript above the atlas’s detection threshold. Anatomy from EMBL-EBI Anatomogram (Apache License 2.0); regions identified by Uberon (uberon.org), CC BY 3.0.

Brain · 10

  • retina23.5 nTPM
  • pituitary gland10.5 nTPM
  • cerebral cortex8.7 nTPM
  • spinal cord7.6 nTPM
  • midbrain5.7 nTPM
  • hypothalamus5.3 nTPM
  • hippocampal formation4.9 nTPM
  • cerebellum4.8 nTPM
  • amygdala4.8 nTPM
  • basal ganglia4.7 nTPM

Peripheral and autonomic · 16

  • thyroid gland29.3 nTPM
  • kidney13.4 nTPM
  • pancreas12.7 nTPM
  • smooth muscle10.7 nTPM
  • heart muscle10.0 nTPM
  • urinary bladder9.7 nTPM
  • lung9.4 nTPM
  • stomach 19.1 nTPM
  • spleen8.9 nTPM
  • colon8.8 nTPM
  • adrenal gland8.6 nTPM
  • salivary gland7.7 nTPM
  • duodenum7.4 nTPM
  • liver7.0 nTPM
  • small intestine6.2 nTPM
  • skeletal muscle5.8 nTPM

Transcript is not protein. These are RNA levels for POLI, shaded on √nTPM so that a three-order range stays legible — on a linear ramp every region but the strongest would read as blank, which looks like absence rather than less. A region carrying no shading is one the atlas reports no consensus value for, not one where the gene is silent. And a gene being transcribed somewhere is not evidence that any compound reaches it. Human Protein Atlas, RNA expression, nTPM (normalized transcripts per million), consensus tissue dataset.

IV · Evidence

Every measurement at this target

Each row links to the ChEMBL assay it came from.

Functional readouts 7

IC₅₀, EC₅₀, AC₅₀ and potency. Assay-dependent, and not interchangeable with a binding constant.

TargetClassTypeValue (nM)pXOrganismAssay
DNA polymerase iotaEnzymesPotency— no normalized value— value does not convert to pXHomo sapiensCHEMBL1794483
DNA polymerase iotaEnzymesPotency— no normalized value— value does not convert to pXHomo sapiensCHEMBL1794483
DNA polymerase iotaEnzymesPotency— no normalized value— value does not convert to pXHomo sapiensCHEMBL1794483
DNA polymerase iotaEnzymesPotency— no normalized value— value does not convert to pXHomo sapiensCHEMBL1794483
DNA polymerase iotaEnzymesPotency— no normalized value— value does not convert to pXHomo sapiensCHEMBL1794483
DNA polymerase iotaEnzymesPotency— no normalized value— value does not convert to pXHomo sapiensCHEMBL1794483
DNA polymerase iotaEnzymesPotency— no normalized value— value does not convert to pXHomo sapiensCHEMBL1794483
← All targets